Molecular Basis of PTP Substrate Recognition and Catalysis
Mechanistic studies with physiological substrates (i.e. phosphoproteins), site-directed mutagenesis, expressed-protein
ligation, X-ray crystallography, H/D exchange-mass spectrometry to probe protein dynamics and interaction.
Activity-based and Interaction Proteomics
Mechanism-based probes to profile PTPs in both normal physiology and pathological conditions. Isolation,
identification, and characterization of PTP substrates/regulators.
Combinatorial chemistry and high throughput screening approaches to acquire
potent and selective PTP inhibitors as tools to modulate PTP functions.
Roles of PTP in Signaling and in Diseases
A number of projects are available to elucidate the roles
of PTPs implicated in diabetes, cancer, and bacterial pathogenesis.